SMURF2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SMURF2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 702-731 of human SMURF2.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
Immnunofluorescence (1:10-1:50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of SMURF2 polyclonal antibody (Cat # PAB1748) in 293 cell line lysate (35 ug/lane). SMURF2 (arrow) was detected using the purified polyclonal antibodyImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SMURF2 polyclonal antibody (Cat # PAB1748) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.Immunofluorescence
Human hippocampal neurons were transfected 2 hrs after plating with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), SMURF2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3)(green). Transfected cells were analyzed at 3 d.i.v. by staining with an SMURF2 polyclonal antibody (Cat # PAB1748)(red). Axons are marked by arrowheads. The marked growth cones are shown at a higher magnification. Scale bars, 40 and 10 µm.
(J. Biol. Chem. 2007 Nov 30;282(48):35259-35268) -
Gene Info — SMURF2
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Interactome
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Pathway
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Publication Reference
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The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity.
Jens C Schwamborn, Mohammad R Khazaei, Andreas W Puschel.
The Journal of Biological Chemistry 2015 Jun; 290(25):15391.
Application:IF, WB-Ce, Rat, Rat embryos, Rat hippocampal neurons.
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The interaction of mPar3 with the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity.
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