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SMURF2 polyclonal antibody

  • Catalog # : PAB1748
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SMURF2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to amino acids 702-731 of human SMURF2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Recommend Usage:
  • Immnunofluorescence (1:10-1:50)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of SMURF2 polyclonal antibody (Cat # PAB1748) in 293 cell line lysate (35 ug/lane). SMURF2 (arrow) was detected using the purified polyclonal antibody
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SMURF2 polyclonal antibody (Cat # PAB1748) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • Immunofluorescence
  • Immunofluorescence
  • Human hippocampal neurons were transfected 2 hrs after plating with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), SMURF2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3)(green). Transfected cells were analyzed at 3 d.i.v. by staining with an SMURF2 polyclonal antibody (Cat # PAB1748)(red). Axons are marked by arrowheads. The marked growth cones are shown at a higher magnification. Scale bars, 40 and 10 µm.
    (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268)
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • SMURF2
  • Gene Alias:
  • DKFZp686F0270,MGC138150
  • Gene Description:
  • SMAD specific E3 ubiquitin protein ligase 2
  • Other Designations:
  • E3 ubiquitin ligase SMURF2
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