ZAK polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, using ZAK polyclonal antibody (Cat # PAB17462).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using ZAK polyclonal antibody (Cat # PAB17462).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ZAK.
Immunogen
A synthetic peptide corresponding to C-terminus of human ZAK.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total ZAK protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, using ZAK polyclonal antibody (Cat # PAB17462).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using ZAK polyclonal antibody (Cat # PAB17462).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — ZAK
Entrez GeneID
51776Protein Accession#
Q9NYL2Gene Name
ZAK
Gene Alias
AZK, MLK7, MLT, MLTK, MRK, mlklak
Gene Description
sterile alpha motif and leucine zipper containing kinase AZK
Omim ID
609479Gene Ontology
HyperlinkGene Summary
This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
MLK-like mitogen-activated protein triple kinase|MLK-related kinase|cervical cancer suppressor gene 4 protein|leucine zipper- and sterile alpha motif-containing kinase|mitogen-activated protein kinase kinase kinase MLT|mixed lineage kinase 7|mixed lineage
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Interactome
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Pathway
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Disease
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