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Last updated: 2016/10/23
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SMURF1 polyclonal antibody

  • Catalog # : PAB1746
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SMURF1.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human SMURF1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The SMURF1 polyclonal antibody (Cat # PAB1746) is used in Western blot to detect SMURF1 in HL-60 cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with SMURF1 polyclonal antibody (Cat # PAB1746) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Gene Name:
  • SMURF1
  • Gene Alias:
  • KIAA1625
  • Gene Description:
  • SMAD specific E3 ubiquitin protein ligase 1
  • Gene Summary:
  • This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. A similar protein in Xenopus is involved in embryonic pattern formation. Alternative splicing results in multiple transcript variants encoding different isoforms. An additional transcript variant has been identified, but its full length sequence has not been determined. [provided by RefSeq
  • Other Designations:
  • E3 ubiquitin ligase SMURF1,Smad ubiquitination regulatory factor 1,Smad-specific E3 ubiquitin ligase 1
  • Interactome
  • Interactome
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