SMG7 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SMG7.
Immunogen
A synthetic peptide corresponding to internal of human SMG7.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total SMG7 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells (Lane 1), Jurkat cells 9Lane 2), 293 cells (Lane 3) and HUVEC cells (Lane 4 and lane 5), using SMG7 polyclonal antibody (Cat # PAB17426).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HepG2 cells, using SMG7 polyclonal antibody (Cat # PAB17426).
Peptide "+" means "with peptide blocking".Enzyme-linked Immunoabsorbent Assay
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Gene Info — SMG7
Entrez GeneID
9887Protein Accession#
Q92540Gene Name
SMG7
Gene Alias
C1orf16, EST1C, FLJ23717, KIAA0250, SGA56M, SMG-7
Gene Description
Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Omim ID
610964Gene Ontology
HyperlinkGene Summary
SMG7 is involved in nonsense-mediated mRNA decay (Fukuhara et al., 2005 [PubMed 15721257]).[supplied by OMIM
Other Designations
EST1 telomerase component homolog C|OTTHUMP00000033244|SMG-7 homolog|breast cancer-associated antigen SGA-56M|ever shorter telomeres 1C
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