AKAP14 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKAP14.
Immunogen
A synthetic peptide corresponding to internal of human AKAP14.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total AKAP14 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells (Lane 1 and lane 4), K-562 cells (Lane 2) and HUVEC cells (Lane 3), using AKAP14 polyclonal antibody (Cat # PAB17405).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using AKAP14 polyclonal antibody (Cat # PAB17405).
Peptide "+" means "with peptide blocking". -
Gene Info — AKAP14
Entrez GeneID
158798Protein Accession#
Q86UN6Gene Name
AKAP14
Gene Alias
AKAP28
Gene Description
A kinase (PRKA) anchor protein 14
Omim ID
300462Gene Ontology
HyperlinkGene Summary
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The protein anchors PKA in ciliary axonemes and, in this way, may play a role in regulating ciliary beat frequency. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
A-kinase anchoring protein 28|OTTHUMP00000023927|OTTHUMP00000023928|OTTHUMP00000023929|protein kinase A anchoring protein 14
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Interactome
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Publication Reference
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, K
Genome Research 2004 Oct; 14(10B):2121.
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
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