MAEA polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using MAEA polyclonal antibody (Cat # PAB17401).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAEA.
Immunogen
A synthetic peptide corresponding to internal of human MAEA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total MAEA protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using MAEA polyclonal antibody (Cat # PAB17401).
Peptide "+" means "with peptide blocking". -
Gene Info — MAEA
Entrez GeneID
10296Protein Accession#
Q7L5Y9Gene Name
MAEA
Gene Alias
EMLP, EMP, HLC-10, PIG5
Gene Description
macrophage erythroblast attacher
Omim ID
606801Gene Ontology
HyperlinkGene Summary
This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq
Other Designations
erythroblast macrophage protein|lung cancer-related protein 10|proliferation-inducing gene 5
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Interactome
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Publication Reference
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Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell division.
Bala S, Kumar A, Soni S, Sinha S, Hanspal M.
Biochemical and Biophysical Research Communications 2006 Feb; 342(4):1040.
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Complete sequencing and characterization of 21,243 full-length human cDNAs.
Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K
Nature Genetics 2003 Dec; 36(1):40.
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Molecular identification and functional characterization of a novel protein that mediates the attachment of erythroblasts to macrophages.
Hanspal M, Smockova Y, Uong Q.
Blood 1998 Oct; 92(8):2940.
Application：WB, Human, Human macrophages.
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Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell division.
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