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Last updated: 2016/12/4
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TAF5 polyclonal antibody

  • Catalog # : PAB17389
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of TAF5.
  • Immunogen:
  • A synthetic peptide corresponding to internal of human TAF5.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Specificity:
  • This antibody detects endogenous levels of total TAF5 protein.
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    Immunohistochemistry (1:50-1:100)
    ELISA (1:5000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from A-549 cells, using TAF5 polyclonal antibody (Cat # PAB17389).
    Peptide "+" means "with peptide blocking".
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using TAF5 polyclonal antibody (Cat # PAB17389).
    Peptide "+" means "with peptide blocking".
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6877
  • Gene Name:
  • TAF5
  • Gene Alias:
  • TAF2D,TAFII100
  • Gene Description:
  • TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa
  • Gene Summary:
  • Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000020402,TATA box binding protein (TBP)-associated factor 2D,TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD,TBP-associated factor 5,transcription initiation factor TFIID 100 kD subunit
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