CSTF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells (Lane 1) and HUVEC cells (Lane 2 and lane 3), using CSTF1 polyclonal antibody (Cat # PAB17377).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CSTF1.
Immunogen
A synthetic peptide corresponding to N-terminus of human CSTF1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total CSTF1 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells (Lane 1) and HUVEC cells (Lane 2 and lane 3), using CSTF1 polyclonal antibody (Cat # PAB17377).
Peptide "+" means "with peptide blocking". -
Gene Info — CSTF1
Entrez GeneID
1477Protein Accession#
Q05048Gene Name
CSTF1
Gene Alias
CstF-50, CstFp50
Gene Description
cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa
Omim ID
600369Gene Ontology
HyperlinkGene Summary
This gene encodes one of three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3'end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq
Other Designations
CF-1 50 kDa subunit|CSTF 50 kDa subunit|OTTHUMP00000031332|cleavage stimulation factor 50kDa subunit|cleavage stimulation factor subunit 1
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Interactome
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Disease
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Publication Reference
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The DNA sequence and comparative analysis of human chromosome 20.
Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafh
Nature 2001 Dec; 414(6866):865.
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Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50.
Kleiman FE, Manley JL.
Science 1999 Sep; 285(5433):1576.
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The human 64-kDa polyadenylylation factor contains a ribonucleoprotein-type RNA binding domain and unusual auxiliary motifs.
Takagaki Y, MacDonald CC, Shenk T, Manley JL.
PNAS 1992 Feb; 89(4):1403.
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The DNA sequence and comparative analysis of human chromosome 20.
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