PKIA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PKIA.
Immunogen
A synthetic peptide corresponding to internal of human PKIA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total PKIA protein.
Form
Liquid
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PKIA polyclonal antibody (Cat # PAB17373).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using PKIA polyclonal antibody (Cat # PAB17373).
Peptide "+" means "with peptide blocking". -
Gene Info — PKIA
Entrez GeneID
5569Protein Accession#
P61925Gene Name
PKIA
Gene Alias
PRKACN1
Gene Description
protein kinase (cAMP-dependent, catalytic) inhibitor alpha
Omim ID
606059Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the cAMP-dependent protein kinase (PKA) inhibitor family. This protein was demonstrated to interact with and inhibit the activities of both C alpha and C beta catalytic subunits of the PKA. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq
Other Designations
PKI-alpha|cAMP-dependent protein kinase inhibitor alpha
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Interactome
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Disease
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Publication Reference
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, K
Genome Research 2004 Oct; 14(10B):2121.
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Inhibition of protein kinase-A by overexpression of the cloned human protein kinase inhibitor.
Olsen SR, Uhler MD.
Molecular Endocrinology 1991 Sep; 5(9):1246.
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
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