HNRNPF polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using HNRNPF polyclonal antibody (Cat # PAB17370).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of HNRNPF.
Immunogen
A synthetic peptide corresponding to N-terminus of human HNRNPF.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total HNRNPF protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using HNRNPF polyclonal antibody (Cat # PAB17370).
Peptide "+" means "with peptide blocking". -
Gene Info — HNRNPF
Entrez GeneID
3185Protein Accession#
P52597Gene Name
HNRNPF
Gene Alias
HNRPF, MGC110997, OK/SW-cl.23, mcs94-1
Gene Description
heterogeneous nuclear ribonucleoprotein F
Omim ID
601037Gene Ontology
HyperlinkGene Summary
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
HnRNP F protein|OTTHUMP00000019482|OTTHUMP00000043413|OTTHUMP00000043414|nucleolin-like protein
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Interactome
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Disease
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Publication Reference
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Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes.
Honore B, Rasmussen HH, Vorum H, Dejgaard K, Liu X, Gromov P, Madsen P, Gesser B, Tommerup N, Celis JE.
The Journal of Biological Chemistry 1995 Dec; 270(48):28780.
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The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.
Matunis MJ, Xing J, Dreyfuss G.
Nucleic Acids Research 1994 Mar; 22(6):1059.
Application：IF, IP, WB-Ce, Human, Yeast, HeLa cells, Yeast cells.
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Identification and characterization of a gene at D10S94 in the MEN2A region.
McDonald H, Smailus D, Jenkins H, Adams K, Simpson NE, Goodfellow PJ.
Genomics 1992 Jun; 13(2):344.
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Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes.
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