Western blot analysis of NEDD8 polyclonal antibody (Cat # PAB1737) in HeLa cell lysate. NEDD8 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEDD8 polyclonal antibody (Cat # PAB1737) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.