NEDD8 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NEDD8.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human NEDD8.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of NEDD8 polyclonal antibody (Cat # PAB1737) in HeLa cell lysate. NEDD8 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with NEDD8 polyclonal antibody (Cat # PAB1737) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — NEDD8
Entrez GeneID
4738Protein Accession#
NP_006147;Q15843Gene Name
NEDD8
Gene Alias
FLJ43224, MGC104393, MGC125896, MGC125897, Nedd-8
Gene Description
neural precursor cell expressed, developmentally down-regulated 8
Omim ID
603171Gene Ontology
HyperlinkGene Summary
O
Other Designations
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Interactome
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Publication Reference
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DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1.
Wu K, Yamoah K, Dolios G, Gan-Erdene T, Tan P, Chen A, Lee CG, Wei N, Wilkinson KD, Wang R, Pan ZQ.
The Journal of Biological Chemistry 2003 Aug; 278(31):28882.
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Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer.
Bohnsack RN, Haas AL.
The Journal of Biological Chemistry 2003 Jul; 278(29):26823.
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The NEDD8 pathway is required for proteasome-mediated degradation of human estrogen receptor (ER)-alpha and essential for the antiproliferative activity of ICI 182,780 in ERalpha-positive breast cancer cells.
Fan M, Bigsby RM, Nephew KP.
Molecular Endocrinology 2003 Mar; 17(3):356.
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DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1.
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