AKAP5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells (Lane 1), HeLa cells (Lane 2) and COLO 205 cells (Lane 3 and lane 4), using AKAP5 polyclonal antibody (Cat # PAB17356).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using AKAP5 polyclonal antibody (Cat # PAB17356).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKAP5.
Immunogen
A synthetic peptide corresponding to N-terminus of human AKAP5.
Host
Rabbit
Reactivity
Human
Specificity
This antibody detects endogenous levels of total AKAP5 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells (Lane 1), HeLa cells (Lane 2) and COLO 205 cells (Lane 3 and lane 4), using AKAP5 polyclonal antibody (Cat # PAB17356).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using AKAP5 polyclonal antibody (Cat # PAB17356).
Peptide "+" means "with peptide blocking". -
Gene Info — AKAP5
Entrez GeneID
9495Protein Accession#
P24588Gene Name
AKAP5
Gene Alias
AKAP75, AKAP79, H21
Gene Description
A kinase (PRKA) anchor protein 5
Omim ID
604688Gene Ontology
HyperlinkGene Summary
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT. [provided by RefSeq
Other Designations
A-kinase anchor protein 5|A-kinase anchor protein, 79kDa|A-kinase anchoring protein 75/79|cAMP-dependent protein kinase regulatory subunit II high affinity binding protein
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Interactome
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Publication Reference
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Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79.
Carr DW, Stofko-Hahn RE, Fraser ID, Cone RD, Scott JD.
The Journal of Biological Chemistry 1992 Aug; 267(24):16816.
Application：WB-Ti, Human, Human brain.
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Cloning and expression of an intron-less gene for AKAP 75, an anchor protein for the regulatory subunit of cAMP-dependent protein kinase II beta.
Hirsch AH, Glantz SB, Li Y, You Y, Rubin CS.
The Journal of Biological Chemistry 1992 Feb; 267(4):2131.
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Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79.
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