MED14 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MED14 polyclonal antibody (Cat # PAB17339).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of COS-7 cells, using MED14 polyclonal antibody (Cat # PAB17339).
Peptide "+" means "with peptide blocking".
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MED14.
Immunogen
A synthetic peptide corresponding to internal of human MED14.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total MED14 protein.
Form
Liquid
Recommend Usage
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using MED14 polyclonal antibody (Cat # PAB17339).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of COS-7 cells, using MED14 polyclonal antibody (Cat # PAB17339).
Peptide "+" means "with peptide blocking". -
Gene Info — MED14
Entrez GeneID
9282Protein Accession#
O60244Gene Name
MED14
Gene Alias
CRSP150, CRSP2, CSRP, CXorf4, DRIP150, EXLM1, MGC104513, RGR1, TRAP170
Gene Description
mediator complex subunit 14
Omim ID
300182Gene Ontology
HyperlinkGene Summary
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq
Other Designations
OTTHUMP00000025778|cofactor required for Sp1 transcriptional activation, subunit 2 (150kD)|cofactor required for Sp1 transcriptional activation, subunit 2, 150kDa|human homolog of yeast RGR1|thyroid hormone receptor-associated protein complex component TR
-
Interactome
-
Disease
-
Publication Reference
-
Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.
Rachez C, Lemon BD, Suldan Z, Bromleigh V, Gamble M, Naar AM, Erdjument-Bromage H, Tempst P, Freedman LP.
Nature 1999 Apr; 398(6730):824.
-
The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1.
Ryu S, Zhou S, Ladurner AG, Tjian R.
Nature 1999 Feb; 397(6718):446.
Application：IP, WB, Human, HeLa cells, Recombinant protein.
-
Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method.
Yoshikawa H, Fujiyama A, Nakai K, Inazawa J, Matsubara K.
Genomics 1998 Apr; 49(2):237.
-
Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com