CREBBP/EP300 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CREBBP/EP300.
Immunogen
A synthetic peptide corresponding to human CREBBP/EP300.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total CREBBP/EP300 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells, using CREBBP/EP300 polyclonal antibody (Cat # PAB17329).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREBBP/EP300 polyclonal antibody (Cat # PAB17329).
Peptide "+" means "with peptide blocking". -
Gene Info — CREBBP
Entrez GeneID
1387Protein Accession#
Q92793 (Gene ID : 1387);Q09472 (Gene ID : 2033)Gene Name
CREBBP
Gene Alias
CBP, KAT3A, RSTS
Gene Description
CREB binding protein
Gene Ontology
HyperlinkGene Summary
This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. First isolated as a nuclear protein that binds to cAMP-response element binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. The protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. This protein acetylates both histone and non-histone proteins. This protein shares regions of very high sequence similarity with protein p300 in its bromodomain, cysteine-histidine-rich regions, and histone acetyltransferase domain. Mutations in this gene cause Rubinstein-Taybi syndrome (RTS). Chromosomal translocations involving this gene have been associated with acute myeloid leukemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
Rubinstein-Taybi syndrome
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Gene Info — EP300
Entrez GeneID
2033Protein Accession#
Q92793 (Gene ID : 1387);Q09472 (Gene ID : 2033)Gene Name
EP300
Gene Alias
KAT3B, p300
Gene Description
E1A binding protein p300
Gene Ontology
HyperlinkGene Summary
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq
Other Designations
E1A-associated protein p300|E1A-binding protein, 300kD|OTTHUMP00000028668|histone acetyltransferase p300
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Loss of CBP acetyltransferase activity by PHD finger mutations in Rubinstein-Taybi syndrome.
Kalkhoven E, Roelfsema JH, Teunissen H, den Boer A, Ariyurek Y, Zantema A, Breuning MH, Hennekam RC, Peters DJ.
Human Molecular Genetics 2003 Feb; 12(4):441.
Application:WB-Ce, Human, Lymphoblastoid cells.
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CBP and p300: versatile coregulators with important roles in hematopoietic gene expression.
Blobel GA.
Journal of Leukocyte Biology 2002 Apr; 71(4):545.
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The HOX homeodomain proteins block CBP histone acetyltransferase activity.
Shen WF, Krishnan K, Lawrence HJ, Largman C.
Mol Cell Biol 2001 Nov; 21(21):7509.
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Loss of CBP acetyltransferase activity by PHD finger mutations in Rubinstein-Taybi syndrome.
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