KRT10 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, using KRT10 polyclonal antibody (Cat # PAB17328).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of A-549 cells, using KRT10 polyclonal antibody (Cat # PAB17328).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KRT10.
Immunogen
A synthetic peptide corresponding to human KRT10.
Host
Rabbit
Reactivity
Human, Rat
Specificity
This antibody detects endogenous levels of total KRT10 protein.
Form
Liquid
Recommend Usage
Western Blot (1:300-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, using KRT10 polyclonal antibody (Cat # PAB17328).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of A-549 cells, using KRT10 polyclonal antibody (Cat # PAB17328).
Peptide "+" means "with peptide blocking". -
Gene Info — KRT10
Entrez GeneID
3858Protein Accession#
P13645Gene Name
KRT10
Gene Alias
CK10, K10, KPP
Gene Description
keratin 10
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq
Other Designations
cytokeratin 10
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Interactome
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Publication Reference
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A human keratin 10 knockout causes recessive epidermolytic hyperkeratosis.
Muller FB, Huber M, Kinaciyan T, Hausser I, Schaffrath C, Krieg T, Hohl D, Korge BP, Arin MJ.
Human Molecular Genetics 2006 Feb; 15(7):1133.
Application：IF, IHC-P, Human, Human skin.
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Out of balance: consequences of a partial keratin 10 knockout.
Reichelt J, Bauer C, Porter R, Lane E, Magin V.
Journal of Cell Science 1997 Sep; 110(Pt 18):2175.
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The complete sequence of the human intermediate filament chain keratin 10. Subdomainal divisions and model for folding of end domain sequences.
Zhou XM, Idler WW, Steven AC, Roop DR, Steinert PM.
The Journal of Biological Chemistry 1988 Oct; 263(30):15584.
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A human keratin 10 knockout causes recessive epidermolytic hyperkeratosis.
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