COL4A1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of COL4A1.
Immunogen
A synthetic peptide corresponding to amino acids 31-70 of human COL4A1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total COL4A1 protein.
Form
Liquid
Recommend Usage
Western Blot (1:300-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, using COL4A1 polyclonal antibody (Cat # PAB17326).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using COL4A1 polyclonal antibody (Cat # PAB17326).
Peptide "+" means "with peptide blocking". -
Gene Info — COL4A1
Entrez GeneID
1282Protein Accession#
P02462Gene Name
COL4A1
Gene Alias
arresten
Gene Description
collagen, type IV, alpha 1
Gene Ontology
HyperlinkGene Summary
This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq
Other Designations
COL4A1 NC1 domain|OTTHUMP00000018698|alpha 1 type IV collagen|collagen IV, alpha-1 polypeptide|collagen of basement membrane, alpha-1 chain|type IV collagen alpha 1
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Interactome
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Pathway
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Disease
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Publication Reference
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The NC1 domain of type IV collagen promotes axonal growth in sympathetic neurons through interaction with the alpha 1 beta 1 integrin.
Lein PJ, Higgins D, Turner DC, Flier LA, Terranova VP.
The Journal of Cell Biology 1991 Apr; 113(2):417.
Application:Func, Rat, Rat sympathetic Neurons.
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Type IV collagen-mediated melanoma cell adhesion and migration: involvement of multiple, distinct domains of the collagen molecule.
Chelberg MK, Tsilibary EC, Hauser AR, McCarthy JB.
Cancer Research 1989 Sep; 49(17):4796.
Application:AFC, Func, Neutralization, Human, K1735 M4 cells.
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Heparin type IV collagen interactions: equilibrium binding and inhibition of type IV collagen self-assembly.
Tsilibary EC, Koliakos GG, Charonis AS, Vogel AM, Reger LA, Furcht LT.
The Journal of Biological Chemistry 1988 Dec; 263(35):19112.
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The NC1 domain of type IV collagen promotes axonal growth in sympathetic neurons through interaction with the alpha 1 beta 1 integrin.
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