APLF polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HT-29 cells, using APLF polyclonal antibody (Cat # PAB17322).
Peptide "+" means "with peptide blocking".
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using APLF polyclonal antibody (Cat # PAB17322).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of APLF.
Immunogen
A synthetic peptide corresponding to residues surrounding serine 116 of human APLF.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total APLF protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HT-29 cells, using APLF polyclonal antibody (Cat # PAB17322).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human brain tissue using APLF polyclonal antibody (Cat # PAB17322).
Peptide "+" means "with peptide blocking". -
Gene Info — APLF
Entrez GeneID
200558Protein Accession#
Q8IW19Gene Name
APLF
Gene Alias
APFL, C2orf13, MGC47799, PALF, Xip1
Gene Description
aprataxin and PNKP like factor
Omim ID
611035Gene Ontology
HyperlinkGene Summary
C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al., 2007 [PubMed 17353262]).[supplied by OMIM
Other Designations
PNK and APTX-like FHA protein|aprataxin- and PNK-like factor
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Interactome
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Publication Reference
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Domain-specific interactions of human HP1-type chromodomain proteins and inner nuclear membrane protein LBR.
Ye Q, Callebaut I, Pezhman A, Courvalin JC, Worman HJ.
The Journal of Biological Chemistry 1997 Jun; 272(23):14983.
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Interaction between an integral protein of the nuclear envelope inner membrane and human chromodomain proteins homologous to Drosophila HP1.
Ye Q, Worman HJ.
The Journal of Biological Chemistry 1996 Jun; 271(25):14653.
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Molecular cloning of a human homologue of Drosophila heterochromatin protein HP1 using anti-centromere autoantibodies with anti-chromo specificity.
Saunders WS, Chue C, Goebl M, Craig C, Clark RF, Powers JA, Eissenberg JC, Elgin SC, Rothfield NF, Earnshaw WC.
Journal of Cell Science 1993 Feb; 104:573.
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Domain-specific interactions of human HP1-type chromodomain proteins and inner nuclear membrane protein LBR.
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