MTOR polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MTOR.
Immunogen
A synthetic peptide corresponding to residues surrounding serine 2448 of human MTOR.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total MTOR protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from K-562 cells, using MTOR polyclonal antibody (Cat # PAB17319).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human brain tissue using MTOR polyclonal antibody (Cat # PAB17319).
Peptide "+" means "with peptide blocking". -
Gene Info — MTOR
Entrez GeneID
2475Protein Accession#
P42345Gene Name
MTOR
Gene Alias
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Gene Description
mechanistic target of rapamycin
Omim ID
601231Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
Other Designations
FK506 binding protein 12-rapamycin associated protein 1|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Thr2446 is a novel mammalian target of rapamycin (mTOR) phosphorylation site regulated by nutrient status.
Cheng SW, Fryer LG, Carling D, Shepherd PR.
The Journal of Biological Chemistry 2004 Apr; 279(16):15719.
Application:WB-Ce, Mouse, CHO-IR cells, H2K myoblasts.
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mTOR-dependent regulation of ribosomal gene transcription requires S6K1 and is mediated by phosphorylation of the carboxy-terminal activation domain of the nucleolar transcription factor UBF.
Hannan KM, Brandenburger Y, Jenkins A, Sharkey K, Cavanaugh A, Rothblum L, Moss T, Poortinga G, McArthur GA, Pearson RB, Hannan RD.
Molecular and Cellular Biology 2003 Dec; 23(23):8862.
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Alcohol impairs leucine-mediated phosphorylation of 4E-BP1, S6K1, eIF4G, and mTOR in skeletal muscle.
Lang CH, Frost RA, Deshpande N, Kumar V, Vary TC, Jefferson LS, Kimball SR.
American Journal of Physiology. Endocrinology and Metabolism 2003 Aug; 285(6):E1205.
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Thr2446 is a novel mammalian target of rapamycin (mTOR) phosphorylation site regulated by nutrient status.
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