Histone H3.1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H3.1.
Immunogen
A synthetic peptide corresponding to residues surrounding serine 10 of human Histone H3.1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total Histone H3.1 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from COLO 205 cells using Histone H3.1 polyclonal antibody (Cat # PAB17318).
Peptide "+" means "with peptide blocking". -
Publication Reference
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The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment.
Dai J, Sultan S, Taylor SS, Higgins JM.
Genes & Development 2005 Jan; 19(4):472.
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Arsenite induces prominent mitotic arrest via inhibition of G2 checkpoint activation in CGL-2 cells.
Yih LH, Hsueh SW, Luu WS, Chiu TH, Lee TC.
Carcinogenesis 2005 Jan; 26(1):53.
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The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment.
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