ATP1A1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, using ATP1A1 polyclonal antibody (Cat # PAB17313).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using ATP1A1 polyclonal antibody (Cat # PAB17313).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATP1A1.
Immunogen
A synthetic peptide corresponding to residues surrounding serine 23 of human ATP1A1.
Host
Rabbit
Reactivity
Human, Rat
Specificity
This antibody detects endogenous levels of total ATP1A1 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HeLa cells, using ATP1A1 polyclonal antibody (Cat # PAB17313).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using ATP1A1 polyclonal antibody (Cat # PAB17313).
Peptide "+" means "with peptide blocking". -
Gene Info — ATP1A1
Entrez GeneID
476Protein Accession#
P06685Gene Name
ATP1A1
Gene Alias
MGC3285, MGC51750
Gene Description
ATPase, Na+/K+ transporting, alpha 1 polypeptide
Omim ID
182310Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
G00-119-711|Na+, K+ ATPase alpha subunit|Na+/K+ -ATPase alpha 1 subunit|Na+/K+ ATPase 1|Na, K-ATPase, alpha-A catalytic polypeptide|Na,K-ATPase alpha-1 subunit|Na,K-ATPase catalytic subunit alpha-A protein|Na/K-ATPase alpha subunit fragment (aa 1-149)|OTT
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Interactome
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Pathway
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Disease
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Publication Reference
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Three differentially expressed Na,K-ATPase alpha subunit isoforms: structural and functional implications.
Herrera VL, Emanuel JR, Ruiz-Opazo N, Levenson R, Nadal-Ginard B.
The Journal of Cell Biology 1987 Oct; 105(4):1855.
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Primary structures of two types of alpha-subunit of rat brain Na+,K+,-ATPase deduced from cDNA sequences.
Hara Y, Urayama O, Kawakami K, Nojima H, Nagamune H, Kojima T, Ohta T, Nagano K, Nakao M.
Journal of Biochemistry 1987 Jul; 102(1):43.
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Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha-subunit from rat brain.
G E Shull, J Greeb, J B Lingrel.
Biochemistry 1986 Dec; 25(25):8125.
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Three differentially expressed Na,K-ATPase alpha subunit isoforms: structural and functional implications.
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