KCNJ3 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, using KCNJ3 polyclonal antibody (Cat # PAB17309).
Peptide "+" means "with peptide blocking".
Immunofluorescence
Immunofluorescence analysis of HeLa cells, using KCNJ3 polyclonal antibody (Cat # PAB17309).
Peptide "+" means "with peptide blocking".
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of KCNJ3.
Immunogen
A synthetic peptide corresponding to residues surrounding serine 185 of human KCNJ3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total KCNJ3 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from NIH/3T3 cells, using KCNJ3 polyclonal antibody (Cat # PAB17309).
Peptide "+" means "with peptide blocking".Immunofluorescence
Immunofluorescence analysis of HeLa cells, using KCNJ3 polyclonal antibody (Cat # PAB17309).
Peptide "+" means "with peptide blocking". -
Gene Info — KCNJ3
Entrez GeneID
3760Protein Accession#
P48549Gene Name
KCNJ3
Gene Alias
GIRK1, KGA, KIR3.1
Gene Description
potassium inwardly-rectifying channel, subfamily J, member 3
Omim ID
601534Gene Ontology
HyperlinkGene Summary
Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. [provided by RefSeq
Other Designations
G protein-activated inward rectifier potassium channel 1|inward rectifier K+ channel KIR3.1|potassium inwardly-rectifying channel J3
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Interactome
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Disease
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Publication Reference
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P, Guyer M, Peck AM, Derge JG, Lipman D, Collins FS, Jang W, Sherry S, Feolo M, Misquitta L, Lee E, Rotmistrovsky K, Greenhut SF, Schaefer CF, Buetow K, Bonner TI, Haussler D, Kent J, Kiekhaus M, Furey T, Brent M, Prange C, Schreiber K, Shapiro N, Bhat NK, Hopkins RF, Hsie F, Driscoll T, Soares MB, Casavant TL, Scheetz TE, Brown-stein MJ, Usdin TB, Toshiyuki S, Carninci P, Piao Y, Dudekula DB, K
Genome Research 2004 Oct; 14(10B):2121.
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Cloning of a G protein-activated inwardly rectifying potassium channel from human cerebellum.
O Schoots, K T Yue, J F MacDonald, D R Hampson, J N Nobrega, L M Dixon, H H Van Tol.
Brain Research. Molecular Brain Research 1996 Jul; 39(1-2):23.
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A recombinant inwardly rectifying potassium channel coupled to GTP-binding proteins.
Chan KW, Langan MN, Sui JL, Kozak JA, Pabon A, Ladias JA, Logothetis DE.
The Journal of General Physiology 1996 Mar; 107(3):381.
Application：IP, WB, Frog, Mouse, CHO cells, Xenopus oocytes.
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The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
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