Histone H4 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H4.
Immunogen
A synthetic peptide corresponding to internal of human Histone H4.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total Histone H4 protein.
Form
Liquid
Recommend Usage
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HepG2 cells, using Histone H4 polyclonal antibody (Cat # PAB17307).
Peptide "+" means "with peptide blocking". -
Publication Reference
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Leaky ribosomal scanning in mammalian genomes: significance of histone H4 alternative translation in vivo.
Smith E, Meyerrose TE, Kohler T, Namdar-Attar M, Bab N, Lahat O, Noh T, Li J, Karaman MW, Hacia JG, Chen TT, Nolta JA, Muller R, Bab I, Frenkel B.
Nucleic Acids Research 2005 Mar; 33(4):1298.
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Yeast chromatin assembly complex 1 protein excludes nonacetylatable forms of histone H4 from chromatin and the nucleus.
Glowczewski L, Waterborg JH, Berman JG.
Molecular and Cellular Biology 2004 Dec; 24(23):10180.
Application:WB-Ce, Yeast, YJB344, YJB347, YJB2861, YJB348.
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Identification of HiNF-P, a key activator of cell cycle-controlled histone H4 genes at the onset of S phase.
Mitra P, Xie RL, Medina R, Hovhannisyan H, Zaidi SK, Wei Y, Harper JW, Stein JL, van Wijnen AJ, Stein GS.
Molecular and Cellular Biology 2003 Nov; 23(22):8110.
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Leaky ribosomal scanning in mammalian genomes: significance of histone H4 alternative translation in vivo.
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