Histone H1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H1.
Immunogen
A synthetic peptide corresponding to residues surrounding threonine 17 of human Histone H1.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total Histone H1 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Histone H1 polyclonal antibody (Cat # PAB17306).
Peptide "+" means "with peptide blocking". -
Publication Reference
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M.
Cell 2006 Nov; 127(3):635.
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The human and mouse replication-dependent histone genes.
Marzluff WF, Gongidi P, Woods KR, Jin J, Maltais LJ.
Genomics 2002 Nov; 80(5):487.
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Characterization of the H1.5 gene completes the set of human H1 subtype genes.
Albig W, Meergans T, Doenecke D.
Gene 1997 Jan; 184(2):141.
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
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