Actin-pan polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Actin-pan.
Immunogen
A synthetic peptide corresponding to residues surrounding tyrosine 53/55 of human Actin-pan.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody detects endogenous levels of total Actin-pan (alpha/beta/gamma) protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
Immunofluorescence (1:500-1:1000)
ELISA (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of extracts from mouse brain cells, using Actin-pan polyclonal antibody (Cat # PAB17300).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using Actin-pan polyclonal antibody (Cat # PAB17300).
Peptide "+" means "with peptide blocking". -
Publication Reference
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PKC-alpha-mediated remodeling of the actin cytoskeleton is involved in constitutive albumin uptake by proximal tubule cells.
Hryciw DH, Pollock CA, Poronnik P.
American Journal of Physiology. Renal Physiology 2005 Jun; 288(6):F1227.
Application:IF, Didelphis marsupialis virginiana, OK cells.
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Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics.
Warren DT, Andrews PD, Gourlay CW, Ayscough KR.
Journal of Cell Science 2002 Apr; 115(Pt 8):1703.
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The integrin alpha6beta4 functions in carcinoma cell migration on laminin-1 by mediating the formation and stabilization of actin-containing motility structures.
Rabinovitz I, Mercurio AM.
The Journal of Cell Biology 1997 Dec; 139(7):1873.
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PKC-alpha-mediated remodeling of the actin cytoskeleton is involved in constitutive albumin uptake by proximal tubule cells.
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