Histone H3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Histone H3.
Immunogen
A synthetic peptide corresponding to residues surrounding serine 363 of human Histone H3.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total Histone H3 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:40000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from A-549 cells, using Histone H3 polyclonal antibody (Cat # PAB17286).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using Histone H3 polyclonal antibody (Cat # PAB17286).
Peptide "+" means "with peptide blocking". -
Publication Reference
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Isolation and characterization of two human H1 histone genes within clusters of core histone genes.
Albig W, Kardalinou E, Drabent B, Zimmer A, Doenecke D.
Genomics 1991 Aug; 10(4):940.
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Enhancer-facilitated expression of prokaryotic and eukaryotic genes using human histone gene 5' regulatory sequences.
Marashi F, Helms S, Shiels A, Silverstein S, Greenspan DS, Stein G, Stein J.
Biochemistry and Cell Biology 1986 Apr; 64(4):277.
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The primary structure and expression of four cloned human histone genes.
Zhong R, Roeder RG, Heintz N.
Nucleic Acids Research 1983 Nov; 11(21):7409.
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Isolation and characterization of two human H1 histone genes within clusters of core histone genes.
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