CEBPA polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CEBPA.
Immunogen
A synthetic peptide corresponding to residues surrounding serine 21 of human CEBPA.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total CEBPA protein.
Form
Liquid
Isotype
IgG
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (150mM NaCl, 0.02% sodium azide, 50% glycerol)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from 293 cells treated with Insulin (0.01 U/mL, 15 mins), using CEBPA polyclonal antibody (Cat # PAB17279).
Peptide "+" means "with peptide blocking".Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using CEBPA polyclonal antibody (Cat # PAB17279).
Peptide "+" means "with peptide blocking". -
Gene Info — CEBPA
Entrez GeneID
1050Protein Accession#
P49715Gene Name
CEBPA
Gene Alias
C/EBP-alpha, CEBP
Gene Description
CCAAT/enhancer binding protein (C/EBP), alpha
Gene Ontology
HyperlinkGene Summary
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. [provided by RefSeq
Other Designations
CCAAT/enhancer binding protein alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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CCAAT/enhancer-binding protein α is epigenetically silenced by histone deacetylation in endometriosis and promotes the pathogenesis of endometriosis.
Kawano Y, Nasu K, Hijiya N, Tsukamoto Y, Amada K, Abe W, Kai K, Moriyama M, Narahara H.
The Journal of Clinical Endocrinology and Metabolism 2013 Sep; 98(9):E1474.
Application:IHC-P, WB-Tr, Human, Endometriotic cyst stromal cells, Endometriotic tissues.
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Expression of CCAAT/enhancer binding proteins alpha and beta in the porcine ovary and regulation in primary cultures of granulosa cells.
Gillio-Meina C, Hui YY, LaVoie HA.
Biology of Reproduction 2005 Jan; 72(5):1194.
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Synergism between ERalpha transactivation function 1 (AF-1) and AF-2 mediated by steroid receptor coactivator protein-1: requirement for the AF-1 alpha-helical core and for a direct interaction between the N- and C-terminal domains.
Metivier R, Penot G, Flouriot G, Pakdel F.
Mol Endocrinol 2001 Nov; 15(11):1953.
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CCAAT/enhancer-binding protein α is epigenetically silenced by histone deacetylation in endometriosis and promotes the pathogenesis of endometriosis.
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