EBF1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EBF1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human EBF1.
Sequence
C-HNQLPALANTSV
Host
Goat
Theoretical MW (kDa)
64.5
Reactivity
Human, Mouse
Specificity
This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
EBF1 polyclonal antibody (Cat # PAB17276) (0.3 ug/mL) staining of human adipose lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — EBF1
Entrez GeneID
1879Protein Accession#
NP_076870.1Gene Name
EBF1
Gene Alias
COE1, EBF, FLJ39389, FLJ41763, O/E-1, OLF1
Gene Description
early B-cell factor 1
Omim ID
164343Gene Ontology
HyperlinkGene Summary
Olf and EBF transcription factor 1|early B-cell factor|olfactory neuronal transcription factor 1
Other Designations
Collier, Olf and EBF transcription factor 1|early B-cell factor|olfactory neuronal transcription factor 1
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Interactome
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Disease
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Publication Reference
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The Cxcl12, periostin, and Ccl9 genes are direct targets for early B-cell factor in OP-9 stroma cells.
Lagergren A, Mansson R, Zetterblad J, Smith E, Basta B, Bryder D, Akerblad P, Sigvardsson M.
The Journal of Biological Chemistry 2007 Mar; 282(19):14454.
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The Cxcl12, periostin, and Ccl9 genes are direct targets for early B-cell factor in OP-9 stroma cells.
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