GAL polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of GAL.
Immunogen
A synthetic peptide corresponding to internal region of human GAL.
Sequence
C-HRSFSDKNGLTSK
Host
Goat
Theoretical MW (kDa)
13.3
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (1-3 ug/mL)
Immunohistochemistry (Frozen sections) (0.2-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
GAL polyclonal antibody (Cat # PAB17271) (1 ug/mL) staining of human cerebellum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Frozen sections)
GAL polyclonal antibody (Cat # PAB17271) (0.3 ug/mL) staining of PFA-perfused cryosection of human hypothalamus. Antigen retrieval with citrate buffer pH 6 at 80C for 30 min, Cy3-staining.
Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.Enzyme-linked Immunoabsorbent Assay
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Gene Info — GAL
Entrez GeneID
51083Protein Accession#
NP_057057.2Gene Name
GAL
Gene Alias
GALN, GLNN, GMAP, MGC40167
Gene Description
galanin prepropeptide
Omim ID
137035Gene Ontology
HyperlinkGene Summary
Galanin is small neuropeptide that functions as a cellular messenger within the central and peripheral nervous systems, modulating diverse physiologic functions (Mechenthaler, 2008 [PubMed 18500643]).[supplied by OMIM
Other Designations
galanin|galanin-message-associated peptide|galanin-related peptide
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Interactome
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Pathway
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Disease
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Publication Reference
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The galanin signaling cascade is a candidate pathway regulating oncogenesis in human squamous cell carcinoma.
Sugimoto T, Seki N, Shimizu S, Kikkawa N, Tsukada J, Shimada H, Sasaki K, Hanazawa T, Okamoto Y, Hata A.
Genes Chromosomes Cancer 2009 Feb; 48(2):132.
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The galanin signaling cascade is a candidate pathway regulating oncogenesis in human squamous cell carcinoma.
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