MIB1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MIB1.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human MIB1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Immunofluorescence (1:20-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
The MIB1 polyclonal antibody (Cat # PAB1726) is used to detect FLAG-tagged MIB1 in 293T cell lysate. Data courtesy of Dr. Olga Ossipova, Mt. Sinai School of Medicine.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MIB1 polyclonal antibody (Cat # PAB1726) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.Immunofluorescence
Immunofluorescent staining of HeLa cells incubated with MIB1 polyclonal antibody (Cat # PAB1726). Data courtesy of Dr. Vyacheslav Akimov, University of Southern Denmark. -
Gene Info — MIB1
Entrez GeneID
57534Protein Accession#
NP_065825;Q86YT6Gene Name
MIB1
Gene Alias
DIP-1, DKFZp686I0769, DKFZp761M1710, FLJ90676, MGC129659, MGC129660, MIB, ZZANK2, ZZZ6
Gene Description
mindbomb homolog 1 (Drosophila)
Omim ID
608677Gene Ontology
HyperlinkOther Designations
DAPK-interacting protein 1|mindbomb homolog 1|ubiquitin ligase mind bomb
-
Interactome
-
Publication Reference
-
Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta.
Itoh M, Kim CH, Palardy G, Oda T, Jiang YJ, Maust D, Yeo SY, Lorick K, Wright GJ, Ariza-McNaughton L, Weissman AM, Lewis J, Chandrasekharappa SC, Chitnis AB.
Developmental Cell 2003 Jan; 4(1):67.
-
A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK.
Jin Y, Blue EK, Dixon S, Shao Z, Gallagher PJ.
The Journal of Biological Chemistry 2002 Sep; 277(49):46980.
Application:IP, WB, Human, Mouse, HeLa cells, Mouse adult tissues, cell lines, embryo .
-
Mind bomb is a ubiquitin ligase that is essential for efficient activation of Notch signaling by Delta.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com