TET2 polyclonal antibody
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More Files
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Flow Cytometry
TET2 polyclonal antibody (Cat # PAB17253) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of TET2.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human TET2.
Sequence
C-PHPQSNNDQQREGSF
Host
Goat
Theoretical MW (kDa)
224, 130
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_001120680.1; NP_060098.3).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:16000)
Flow Cytometry (10 ug/mL)
Immunohistochemistry (2-6 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
TET2 polyclonal antibody (Cat # PAB17253) Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Gene Info — TET2
Entrez GeneID
54790Protein Accession#
NP_001120680.1;NP_060098.3Gene Name
TET2
Gene Alias
FLJ20032, KIAA1546, MGC125715
Gene Description
tet oncogene family member 2
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000161869
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Interactome
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Disease
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Publication Reference
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Acquired mutations in TET2 are common in myelodysplastic syndromes.
Langemeijer SM, Kuiper RP, Berends M, Knops R, Aslanyan MG, Massop M, Stevens-Linders E, van Hoogen P, van Kessel AG, Raymakers RA, Kamping EJ, Verhoef GE, Verburgh E, Hagemeijer A, Vandenberghe P, de Witte T, van der Reijden BA, Jansen JH.
Nature Genetics 2009 May; 41(7):838.
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Acquired mutations in TET2 are common in myelodysplastic syndromes.
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