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Last updated: 2016/11/27
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NEFM polyclonal antibody

  • Catalog # : PAB17252
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  • Specification
  • Product Description:
  • Goat polyclonal antibody raised against synthetic peptide of NEFM.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids at internal region of human NEFM.
  • Sequence:
  • C-QASHITVERKDYLK
  • Host:
  • Goat
  • Theoretical MW (kDa):
  • 102, 59.5
  • Reactivity:
  • Mouse
  • Specificity:
  • This antibody is expected to recognize both reported isoforms (NP_005373.2; NP_001099011.1).
  • Form:
  • Liquid
  • Purification:
  • Antigen affinity purification
  • Concentration:
  • 0.5 mg/mL
  • Recommend Usage:
  • ELISA (1:128000)
    Western Blot (0.03-0.1 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • NEFM polyclonal antibody (Cat # PAB17252) (0.03 ug/mL) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
  • ELISA
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 4741
  • Protein Accession#:
  • NP_005373.2;NP_001099011.1
  • Gene Name:
  • NEFM
  • Gene Alias:
  • NEF3,NF-M,NFM
  • Gene Description:
  • neurofilament, medium polypeptide
  • Gene Summary:
  • Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
  • Other Designations:
  • neurofilament triplet M protein,neurofilament, medium polypeptide 150kDa,neurofilament-3 (150 kD medium)
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