Rabbit polyclonal antibody raised against synthetic peptide of COL1A1.
A synthetic peptide corresponding to human COL1A1.
Theoretical MW (kDa):
Birds, Fish, Human, Mammals, Mouse, Rat
This antibody has been directly tested for reactivity in human, mouse and rat. Based on the homology of the antigen this antibody is expected to recognize the collagen I 1 polypeptide in most, if not all, species of mammals, birds, amphibians, and fishes. specific to the propeptide portion of the ~180 KDa collagen I alpha1 polypeptide in human lung fibroblast extract. This antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90°C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, This antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
Western Blot (1:1000) Immunohistochemistry (1:100) The optimal working dilution should be determined by the end user.
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA)
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Western blot of rat lung lysate showing specific immunolabeling of the ~180 k COL1A1 protein. Using COL1A1 polyclonal antibody (Cat # PAB17204).
Immunofluorescence staining of fibrotic mouse lung tissue showing specific staining of COL1A1 molecules (red) that are still associated with the cells in which they were synthesized. Using COL1A1 polyclonal antibody (Cat # PAB17204).
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish
alpha 1 type I collagen,collagen alpha 1 chain type I,collagen of skin, tendon and bone, alpha-1 chain,pro-alpha-1 collagen type 1,type I collagen alpha 1 chain