COL1A1 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of rat lung lysate showing specific immunolabeling of the ~180 k COL1A1 protein. Using COL1A1 polyclonal antibody (Cat # PAB17204).
Immunofluorescence
Immunofluorescence staining of fibrotic mouse lung tissue showing specific staining of COL1A1 molecules (red) that are still associated with the cells in which they were synthesized. Using COL1A1 polyclonal antibody (Cat # PAB17204).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of COL1A1.
Immunogen
A synthetic peptide corresponding to human COL1A1.
Host
Rabbit
Theoretical MW (kDa)
180
Reactivity
Birds, Fish, Human, Mammals, Mouse, Rat
Specificity
This antibody has been directly tested for reactivity in human, mouse and rat. Based on the homology of the antigen this antibody is expected to recognize the collagen I 1 polypeptide in most, if not all, species of mammals, birds, amphibians, and fishes. specific to the propeptide portion of the ~180 KDa collagen I alpha1 polypeptide in human lung fibroblast extract. This antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90°C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20). Note that in paraffin sections of formaldehyde-fixed fibrotic mouse lung tissue, This antibody recognizes collagen I molecules that are still associated with the cells in which they were synthesized.
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 0.1 mg/mL BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat lung lysate showing specific immunolabeling of the ~180 k COL1A1 protein. Using COL1A1 polyclonal antibody (Cat # PAB17204).Immunohistochemistry
Immunofluorescence
Immunofluorescence staining of fibrotic mouse lung tissue showing specific staining of COL1A1 molecules (red) that are still associated with the cells in which they were synthesized. Using COL1A1 polyclonal antibody (Cat # PAB17204). -
Gene Info — COL1A1
Entrez GeneID
1277Protein Accession#
P02452Gene Name
COL1A1
Gene Alias
OI4
Gene Description
collagen, type I, alpha 1
Gene Ontology
HyperlinkGene Summary
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish
Other Designations
alpha 1 type I collagen|collagen alpha 1 chain type I|collagen of skin, tendon and bone, alpha-1 chain|pro-alpha-1 collagen type 1|type I collagen alpha 1 chain
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Interactome
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Pathway
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Disease
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Publication Reference
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Mutations near amino end of alpha1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing.
Cabral WA, Makareeva E, Colige A, Letocha AD, Ty JM, Yeowell HN, Pals G, Leikin S, Marini JC.
The Journal of Biological Chemistry 2005 May; 280(19):19259.
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Inherited disorders of collagen gene structure and expression.
Byers PH.
American Journal of Medical Genetics 1989 Sep; 34(1):72.
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Mutations near amino end of alpha1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing.
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