ch-TOG polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot on human HeLa whole cell lysate (WCL) and ch-TOG immunoprecipitated (I) from mouse embryonic fibroblast whole cell lysate. The ch-TOG polyclonal antibody (Cat # PAB17111) did not react with the mouse embryonic fibroblast whole cell lysate when non-immune sera (NI) was used in the immunoprecipitation reaction. Proteins were resolved and transferred from a 9% SDS-PAGE.
Immunofluorescence
Immunofluorescent images (100X) of a mouse fibroblast cell line labeled with ch-TOG (red), microtubules (green) demonstrating ch-TOG localizes with microtubules (yellow). Using ch-TOG polyclonal antibody (Cat # PAB17111).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ch-TOG.
Immunogen
A synthetic peptide corresponding to C-terminus amino acids of ch-TOG.
Host
Rabbit
Reactivity
Bovine, Chicken, Chimpanzee, Dog, Frog, Human, Mouse, Opossum, Rat
Specificity
This antibody recognizes human and mouse ch-TOG. A BLAST search demonstrated 100% identity to the peptide immunogen in human, mouse, rat, chimpanzee, dog, cow, chicken, opossum, and frog.
Form
Liquid
Recommend Usage
Western Blot (1:5000-1:10000)
Immunoprecipitation (1:5000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot on human HeLa whole cell lysate (WCL) and ch-TOG immunoprecipitated (I) from mouse embryonic fibroblast whole cell lysate. The ch-TOG polyclonal antibody (Cat # PAB17111) did not react with the mouse embryonic fibroblast whole cell lysate when non-immune sera (NI) was used in the immunoprecipitation reaction. Proteins were resolved and transferred from a 9% SDS-PAGE.Immunofluorescence
Immunofluorescent images (100X) of a mouse fibroblast cell line labeled with ch-TOG (red), microtubules (green) demonstrating ch-TOG localizes with microtubules (yellow). Using ch-TOG polyclonal antibody (Cat # PAB17111).Immunoprecipitation
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