COX4I1/COX4I2 polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
COX4I1/COX4I2 polyclonal antibody (Cat # PAB17042) (1 ug/mL) staining of human skeletal muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of COX4I1/COX4I2.
Immunogen
A synthetic peptide corresponding to human COX4I1/COX4I2.
Sequence
C-RWDYEKKQWKK
Host
Goat
Theoretical MW (kDa)
19.6
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:2000)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
COX4I1/COX4I2 polyclonal antibody (Cat # PAB17042) (1 ug/mL) staining of human skeletal muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — COX4I1
Entrez GeneID
1327Protein Accession#
NP_115998.2Gene Name
COX4I1
Gene Alias
COX4, COXIV, MGC72016
Gene Description
cytochrome c oxidase subunit IV isoform 1
Omim ID
123864Gene Ontology
HyperlinkGene Summary
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq
Other Designations
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Gene Info — COX4I2
Entrez GeneID
84701Protein Accession#
NP_115998.2Gene Name
COX4I2
Gene Alias
COX4, COX4-2, COX4B, COX4L2, COXIV-2, dJ857M17.2
Gene Description
cytochrome c oxidase subunit IV isoform 2 (lung)
Omim ID
607976Gene Ontology
HyperlinkGene Summary
Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation. [provided by RefSeq
Other Designations
OTTHUMP00000030533|cytochrome c oxidase subunit IV isoform 2|cytochrome c oxidase subunit IV-like 2
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Interactome
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Pathway
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Disease
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Publication Reference
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HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells.
Fukuda R, Zhang H, Kim JW, Shimoda L, Dang CV, Semenza GL.
Cell 2007 Apr; 129(1):111.
Application：WB, Human, Mouse, HeLa cells, Mouse embryo fibroblasts.
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HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells.
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