UBE2F polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of UBE2F.
Immunogen
A synthetic peptide corresponding to C terminus of human UBE2F.
Sequence
C-HLRDKEDFRNKVDD
Host
Goat
Theoretical MW (kDa)
21.1
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.05-0.2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5-3.8 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
UBE2F polyclonal antibody (Cat # PAB17034) (0.05 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
UBE2F polyclonal antibody (Cat # PAB17034) staining of paraffin embedded human placenta.Enzyme-linked Immunoabsorbent Assay
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Gene Info — UBE2F
Entrez GeneID
140739Protein Accession#
NP_542409.1Gene Name
UBE2F
Gene Alias
MGC18120, NCE2
Gene Description
ubiquitin-conjugating enzyme E2F (putative)
Gene Ontology
HyperlinkOther Designations
NEDD8 conjugating enzyme|NEDD8-conjugating enzyme
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Interactome
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Pathway
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Publication Reference
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E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA.
Molecular Cell 2009 Feb; 33(4):483.
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E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
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