MEST polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
MEST polyclonal antibody (Cat # PAB17021) (0.2 ug/mL) staining of mouse testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MEST.
Immunogen
A synthetic peptide corresponding to human MEST.
Sequence
C-QELLYRYKQNRSGR
Host
Goat
Theoretical MW (kDa)
38.8, 37.6
Reactivity
Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:8000)
Western Blot (0.2-0.5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
MEST polyclonal antibody (Cat # PAB17021) (0.2 ug/mL) staining of mouse testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MEST
Entrez GeneID
4232Protein Accession#
NP_002393.2;NP_803490.1Gene Name
MEST
Gene Alias
DKFZp686L18234, MGC111102, MGC8703, PEG1
Gene Description
mesoderm specific transcript homolog (mouse)
Omim ID
601029Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the [alpha]/[beta] hydrolase fold family and has isoform specific imprinting. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Three transcript variants encoding two distinct isoforms have been identified for this gene. A pseudogene for this locus is located on chromosome 6. [provided by RefSeq
Other Designations
mesoderm specific transcript|paternally expressed gene 1
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Interactome
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Disease
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Publication Reference
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An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa.
Li T, Vu TH, Lee KO, Yang Y, Nguyen CV, Bui HQ, Zeng ZL, Nguyen BT, Hu JF, Murphy SK, Jirtle RL, Hoffman AR.
The Journal of Biological Chemistry 2002 Apr; 277(16):13518.
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An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa.
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