UBE2S polyclonal antibody
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More Files
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Western Blot (Cell lysate)
UBE2S polyclonal antibody (Cat # PAB1702) is used in Western blot to detect UBE2S in HL-60 cell lysate.
Western Blot (Transfected lysate)
Western blot analysis of UBE2S (arrow) using UBE2S polyclonal antibody (Cat # PAB1702). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2S polyclonal antibody (Cat # PAB1702) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBE2S.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human UBE2S.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
UBE2S polyclonal antibody (Cat # PAB1702) is used in Western blot to detect UBE2S in HL-60 cell lysate.Western Blot (Transfected lysate)
Western blot analysis of UBE2S (arrow) using UBE2S polyclonal antibody (Cat # PAB1702). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the UBE2S gene (Lane 2) (Origene Technologies).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with UBE2S polyclonal antibody (Cat # PAB1702) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — UBE2S
Entrez GeneID
27338Protein Accession#
NP_055316;Q16763Gene Name
UBE2S
Gene Alias
E2-EPF, E2EPF, EPF5
Gene Description
ubiquitin-conjugating enzyme E2S
Omim ID
610309Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the ubiquitin-conjugating enzyme family. The encoded protein is able to form a thiol ester linkage with ubiquitin in a ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. [provided by RefSeq
Other Designations
ubiquitin carrier protein S|ubiquitin-conjugating enzyme E2-24 kD|ubiquitin-protein ligase S
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Interactome
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Pathway
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Publication Reference
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA,
PNAS 2002 Dec; 99(26):16899.
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cDNA cloning of a novel human ubiquitin carrier protein. An antigenic domain specifically recognized by endemic pemphigus foliaceus autoantibodies is encoded in a secondary reading frame of this human epidermal transcript.
Liu Z, Diaz LA, Haas AL, Giudice GJ.
The Journal of Biological Chemistry 1992 Aug; 267(22):15829.
Application：WB-Re, Recombinant protein.
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Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
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