GST polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant GST.
Immunogen
Recombinant protein corresponding to bacteria GST.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes over-expressed proteins containing GST epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
Form
Liquid
Recommend Usage
Western Blot (1:1000-1:4000)
Immunoprecipitation (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
GST Western blot analysis of 293 cells transfected with GST-tagged vector (Lane 1) and untransfected control (Lane 2) using GST polyclonal antibody (Cat # PAB17015).Immunoprecipitation
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Publication Reference
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Baculovirus-mediated expression and isolation of human ribosomal phosphoprotein P0 carrying a GST-tag in a functional state.
Abo Y, Hagiya A, Naganuma T, Tohkairin Y, Shiomi K, Kajiura Z, Hachimori A, Uchiumi T, Nakagaki M.
Biochemical and Biophysical Research Communications 2004 Sep; 322(3):814.
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Baculovirus-mediated expression and isolation of human ribosomal phosphoprotein P0 carrying a GST-tag in a functional state.
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