V5 tag polyclonal antibody
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptides of V5 tag.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 95-108 of V5 tag.
Sequence
GKPIPNPLLGLDST
Host
Goat
Reactivity
Human, Mouse, Rat
Form
Liquid
Recommend Usage
Western Blot (1:1000-1:10000)
ELISA (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of 293 cells transfected with a lacZ-V5-tagged vector (Lane 2) and untransfected control (Lane 1). Detection with V5 tag polyclonal antibody (Cat # PAB17014).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Molecular cloning and characterization of canine pre-B-cell colony-enhancing factor.
McGlothlin JR, Gao L, Lavoie T, Simon BA, Easley RB, Ma SF, Rumala BB, Garcia JG, Ye SQ.
Biochemical Genetics 2005 Apr; 43(3-4):127.
Application:IF, WB-Tr, Human, A549 cells.
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Molecular cloning and characterization of canine pre-B-cell colony-enhancing factor.
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