Crk2 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using Crk2 polyclonal antibody (Cat # PAB17003).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Crk2.
Immunogen
A synthetic peptide corresponding to residues surrounding Y221 of human Crk2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of total Crk2 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using Crk2 polyclonal antibody (Cat # PAB17003).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain.
Ballif BA, Carey GR, Sunyaev SR, Gygi SP.
Journal of Proteome Research 2008 Jan; 7(1):311.
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The C-terminal SH3 domain of the mouse c-Crk protein negatively regulates tyrosine-phosphorylation of Crk associated p130 in rat 3Y1 cells.
Ogawa S, Toyoshima H, Kozutsumi H, Hagiwara K, Sakai R, Tanaka T, Hirano N, Mano H, Yazaki Y, Hirai H.
Oncogene 1994 Jun; 9(6):1669.
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Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain.
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