CHEK1 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CHEK1.
Immunogen
A synthetic peptide corresponding to residues surrounding S317 of human CHEK1.
Host
Rabbit
Reactivity
Human, Rat
Specificity
This antibody detects endogenous levels of total CHEK1 protein.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:20000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHEK1 polyclonal antibody (Cat # PAB17001).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CHEK1
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Interactome
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Pathway
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Disease
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Publication Reference
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A quantitative atlas of mitotic phosphorylation.
Dephoure N, Zhou C, Villen J, Beausoleil SA, Bakalarski CE, Elledge SJ, Gygi SP.
PNAS 2008 Jul; 105(31):10762.
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Enhanced DNA-PK-mediated RPA2 hyperphosphorylation in DNA polymerase eta-deficient human cells treated with cisplatin and oxaliplatin.
Cruet-Hennequart S, Glynn MT, Murillo LS, Coyne S, Carty MP.
DNA Repair 2008 Apr; 7(4):582.
Application:WB-Ce, Human, GM00637, XP30RO cells.
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ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ.
Science 2007 May; 316(5828):1160.
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A quantitative atlas of mitotic phosphorylation.
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