Crk2 (phospho Y221) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of Crk2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Y221 of human Crk2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Crk2 only when phosphorylated at tyrosine 221.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using Crk2 (phospho Try221) polyclonal antibody (Cat # PAB16967).Enzyme-linked Immunoabsorbent Assay
-
Publication Reference
-
Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain.
Ballif BA, Carey GR, Sunyaev SR, Gygi SP.
Journal of Proteome Research 2008 Jan; 7(1):311.
-
The C-terminal SH3 domain of the mouse c-Crk protein negatively regulates tyrosine-phosphorylation of Crk associated p130 in rat 3Y1 cells.
Ogawa S, Toyoshima H, Kozutsumi H, Hagiwara K, Sakai R, Tanaka T, Hirano N, Mano H, Yazaki Y, Hirai H.
Oncogene 1994 Jun; 9(6):1669.
-
Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com