CHEK2 (phospho T387) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T387 of human CHEK2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of CHEK2 only when phosphorylated at serine 317.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot of Jurkat cells using CHEK2 (phospho Thr387) polyclonal antibody (Cat # PAB16966).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHEK2 (phospho Thr387) polyclonal antibody (Cat # PAB16966).Enzyme-linked Immunoabsorbent Assay
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Gene Info — CHEK2
Entrez GeneID
11200Protein Accession#
O96017Gene Name
CHEK2
Gene Alias
CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53
Gene Description
CHK2 checkpoint homolog (S. pombe)
Gene Ontology
HyperlinkGene Summary
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000028871|checkpoint-like protein CHK2|protein kinase CHK2|serine/threonine-protein kinase CHK2
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Interactome
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Publication Reference
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Autophosphorylated residues involved in the regulation of human chk2 in vitro.
Gabant G, Lorphelin A, Nozerand N, Marchetti C, Bellanger L, Dedieu A, Quemeneur E, Alpha-Bazin B.
Journal of Molecular Biology 2008 Jul; 380(3):489.
Application:WB, Sf9 insect cells, Sf9 insect cells.
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Human T-cell leukemia virus type 1 Tax oncoprotein prevents DNA damage-induced chromatin egress of hyperphosphorylated Chk2.
Gupta SK, Guo X, Durkin SS, Fryrear KF, Ward MD, Semmes OJ.
The Journal of Biological Chemistry 2007 Oct; 282(40):29431.
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The Wip1 phosphatase (PPM1D) antagonizes activation of the Chk2 tumour suppressor kinase.
Oliva-Trastoy M, Berthonaud V, Chevalier A, Ducrot C, Marsolier-Kergoat MC, Mann C, Leteurtre F.
Oncogene 2007 Mar; 26(10):1449.
Application:WB-Tr, Hamster, CHO cells.
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Autophosphorylated residues involved in the regulation of human chk2 in vitro.
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