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Last updated: 2017/9/17
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FOXO3 (phospho S253) polyclonal antibody

  • Catalog # : PAB16950
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of FOXO3.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S253 of human FOXO3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody detects endogenous levels of FOXO3 only when phosphorylated at serine 253.
  • Form:
  • Liquid
  • Recommend Usage:
  • Western Blot (1:500-1:1000)
    Immunohistochemistry (1:50-1:100)
    ELISA (1:4000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C for three months. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis of extracts from NIH/3T3 cells treated with using Serum.
    Lane 1 : Using FOXO3 (phospho S253) polyclonal antibody (Cat # PAB16950).
    Lane 2 : Using the same antibody preincubated with synthesized peptide.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOXO3 (phospho S253) polyclonal antibody (Cat # PAB16950).
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2309
  • Gene Name:
  • FOXO3
  • Gene Alias:
  • AF6q21,DKFZp781A0677,FKHRL1,FKHRL1P2,FOXO2,FOXO3A,MGC12739,MGC31925
  • Gene Description:
  • forkhead box O3
  • Gene Summary:
  • This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000016944,forkhead box O3A,forkhead homolog (rhabdomyosarcoma) like 1,forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
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