ERK1/2 (phospho T202/Y204) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERK1/2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding T202/Y204 of human ERK1/2.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of ERK1/2 only when phosphorylated at Thr202/Tyr204.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells treated with PMA.
Lane 1 : Using ERK1/2 (phospho T202/Y204) polyclonal antibody (Cat # PAB16949).
Lane 2 : Using the same antibody preincubated with synthesized peptide.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ERK1/2 (phospho T202/Y204) polyclonal antibody (Cat # PAB16949).Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Differential regulation of MAP kinase signalling by dual-specificity protein phosphatases.
Owens DM, Keyse SM.
Oncogene 2007 May; 26(22):3203.
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Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer.
Roberts PJ, Der CJ.
Oncogene 2007 May; 26(22):3291.
Application:WB-Tr, Human, Mammalian cells.
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Identification of regulatory phosphorylation sites in mitogen-activated protein kinase (MAPK)-activated protein kinase-1a/p90rsk that are inducible by MAPK.
Dalby KN, Morrice N, Caudwell FB, Avruch J, Cohen P.
The Journal of Biological Chemistry 1998 Jan; 273(3):1496.
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Differential regulation of MAP kinase signalling by dual-specificity protein phosphatases.
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