PRKAB1 (phospho S182) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells.
Lane 1 : Using PRKAB1 (phospho S182) polyclonal antibody (Cat # PAB16948).
Lane 2 : Using the same antibody preincubated with synthesized peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PRKAB1 (phospho S182) polyclonal antibody (Cat # PAB16948).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of PRKAB1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S182 of human PRKAB1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of PRKAB1 only when phosphorylated at serine 182.
Form
Liquid
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
ELISA (1:4000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM PBS, 0.15 M NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C for three months. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from Jurkat cells.
Lane 1 : Using PRKAB1 (phospho S182) polyclonal antibody (Cat # PAB16948).
Lane 2 : Using the same antibody preincubated with synthesized peptide.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PRKAB1 (phospho S182) polyclonal antibody (Cat # PAB16948).Enzyme-linked Immunoabsorbent Assay
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Gene Info — PRKAB1
Entrez GeneID
5564Protein Accession#
Q9Y478Gene Name
PRKAB1
Gene Alias
AMPK, HAMPKb, MGC17785
Gene Description
protein kinase, AMP-activated, beta 1 non-catalytic subunit
Omim ID
602740Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq
Other Designations
5'-AMP-activated protein kinase beta-1 subunit|AMP-activated protein kinase beta 1 non-catalytic subunit|AMP-activated protein kinase beta subunit|AMPK beta -1 chain|AMPK beta 1|protein kinase, AMP-activated, noncatalytic, beta-1
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Interactome
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Pathway
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Disease
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Publication Reference
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The AMP-activated protein kinase pathway--new players upstream and downstream.
Hardie DG.
Journal of Cell Science 2004 Nov; 117(Pt 23):5479.
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The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress.
Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, Cantley LC.
PNAS 2004 Feb; 101(10):3329.
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Role of AMP-activated protein kinase in cyclic AMP-dependent lipolysis In 3T3-L1 adipocytes.
Yin W, Mu J, Birnbaum MJ.
The Journal of Biological Chemistry 2003 Oct; 278(44):43074.
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The AMP-activated protein kinase pathway--new players upstream and downstream.
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