Postn polyclonal antibody
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More Files
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Western Blot (Tissue lysate)
Western blot of rat lung lysate showing specific immunolabeling of the ~ 93 kDa Postn protein triplet.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of Postn.
Immunogen
A synthetic peptide corresponding to fasciclin domain 1 of mouse Postn.
Host
Rabbit
Theoretical MW (kDa)
93
Reactivity
Chicken, Human, Mouse, Rat
Specificity
This antibody has been directly tested for reactivity in chicken, human, mouse and rat. Based on the homology of the immunogen with other species, we expect that this antibody will recognize periostin in most, if not all, species of mammals, birds, amphibians, and fishes. specific to three periostin bands around ~93 KDa in mouse lung extract. The immunogenic peptide is present in all known splice variants of periostin and therefore this antibody recognizes all known molecular forms of periostin. This antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigenretrieval protocol (incubate slides for 20 minutes at 90 °C in 10 mM sodium citrate (pH 6.0)/ 0.1% Tween-20).
Form
Liquid
Purification
Affinity purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of rat lung lysate showing specific immunolabeling of the ~ 93 kDa Postn protein triplet.Immunohistochemistry
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Gene Info — Postn
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Publication Reference
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Periostin modulates extracellular matrix behavior in tendons.
Kevin I Rolnick, Joshua A Choe, Ellen M Leiferman, Jaclyn Kondratko-Mittnacht, Anna E B Clements, Geoffrey S Baer, Peng Jiang, Ray Vanderby, Connie S Chamberlain.
Matrix Biology Plus 2022 Dec; 16:100124.
Application：IHC, Mouse, Mouse achilles tendon.
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Neonatal and adult cardiovascular pathophysiological remodeling and repair: developmental role of periostin.
Norris RA, Borg TK, Butcher JT, Baudino TA, Banerjee I, Markwald RR.
Annals of the New York Academy of Sciences 2008 Mar; 1123:30.
Application：WB-Ce, Mouse, Mouse cardiac mesenchymal cells.
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Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues.
Norris RA, Damon B, Mironov V, Kasyanov V, Ramamurthi A, Moreno-Rodriguez R, Trusk T, Potts JD, Goodwin RL, Davis J, Hoffman S, Wen X, Sugi Y, Kern CB, Mjaatvedt CH, Turner DK, Oka T, Conway SJ, Molkentin JD, Forgacs G, Markwald RR.
Journal of Cellular Biochemistry 2007 Jun; 101(3):695.
Application：EM, IHC, IP-WB, WB, Human, Mouse, Heart, Skin, HEK293 cells.
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Periostin is expressed within the developing teeth at the sites of epithelial-mesenchymal interaction.
Kruzynska-Frejtag A, Wang J, Maeda M, Rogers R, Krug E, Hoffman S, Markwald RR, Conway SJ.
Developmental Dynamics 2004 Apr; 229(4):857.
Application：IHC, WB-Ti, Mouse, Mouse cervical loop, Tooth.
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Periostin modulates extracellular matrix behavior in tendons.
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