Rabbit polyclonal antibody raised against partial recombinant Postn.
Recombinant protein corresponding to 188 amino acids from the C-terminus of mouse Postn.
Theoretical MW (kDa):
Birds, Chicken, Human, Mammals, Mouse, Rat
This antibody has been directly tested for reactivity in chicken, human, mouse and rat. This antibody is expected to work on all mammalian and avian species. specific to the ~93 KDa periostin doublet in mouse lung extract. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes only the largest two of the three forms of periostin recognized on Western blots by the Pan periostin antibody and also shows a distinctive staining pattern by immunohistochemistry. This antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 °C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20).
Western Blot (1:1000) Immunohistochemistry (1:100) The optimal working dilution should be determined by the end user.
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.