Olig2 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~ 32k Olig2 protein.
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Specifications
Product Description
Rabbit polyclonal antibody raised against recombinant Olig2.
Immunogen
Recombinant protein corresponding to mouse Olig2.
Host
Rabbit
Theoretical MW (kDa)
27
Reactivity
Human, Mouse, Rat
Specificity
This antibody has been directly tested for reactivity in human, mouse and rat. specific to the ~32 KDa Olig2 protein in Western blots. This antibody also works well for immunohistochemistry and immunocytochemistry.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Western Blot (1:3000)
Immunohistochemistry (1:1000)
Immunocytochemistry (1:1000)
Immunoprecipitation (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 200 mM Tris, 100 mM glycine, pH 8.0
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~ 32k Olig2 protein.Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
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Gene Info — Olig2
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Publication Reference
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Olig2-regulated lineage-restricted pathway controls replication competence in neural stem cells and malignant glioma.
Ligon KL, Huillard E, Mehta S, Kesari S, Liu H, Alberta JA, Bachoo RM, Kane M, Louis DN, Depinho RA, Anderson DJ, Stiles CD, Rowitch DH.
Neuron 2007 Feb; 53(4):503.
Application：ChIP, Flow Cyt, IHC-P, Human, Rat, Human GBM tissues, glioma cells HOG, Rat cortical progenitor cells.
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bHLH transcription factor Olig1 is required to repair demyelinated lesions in the CNS.
Arnett HA, Fancy SP, Alberta JA, Zhao C, Plant SR, Kaing S, Raine CS, Rowitch DH, Franklin RJ, Stiles CD.
Science 2004 Dec; 306(5704):2111.
Application：IF, IHC, Mouse, Mouse brain.
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Olig2-regulated lineage-restricted pathway controls replication competence in neural stem cells and malignant glioma.
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