Olig1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant Olig1.
Immunogen
Recombinant protein corresponding to Olig1.
Host
Rabbit
Theoretical MW (kDa)
27
Reactivity
Mouse, Rat
Specificity
This antibody has been directly tested for reactivity in rat and mouse. specific to the ~27 KDa Olig1 protein in Western blots. This antibody also works well for immunohistochemistry, immunocytochemistry and immunoprecipitation.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Western Blot (1:3000)
Immunohistochemistry (1:1000)
Immunocytochemistry (1:1000)
Immunoprecipitation (1:50)
The optimal working dilution should be determined by the end user.Storage Buffer
In 200 mM Tris, 100 mM glycine, pH 8.0
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Tissue lysate)
Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~ 27k Olig1 protein.Immunohistochemistry
Immunocytochemistry
Immunoprecipitation
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Publication Reference
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bHLH transcription factor Olig1 is required to repair demyelinated lesions in the CNS.
Arnett HA, Fancy SP, Alberta JA, Zhao C, Plant SR, Kaing S, Raine CS, Rowitch DH, Franklin RJ, Stiles CD.
Science 2004 Dec; 306(5704):2111.
Application:IF, IHC, Mouse, Mouse brain.
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Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors.
Zhou Q, Wang S, Anderson DJ.
Neuron 2000 Feb; 25(2):331.
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bHLH transcription factor Olig1 is required to repair demyelinated lesions in the CNS.
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